{"title":"P2250 EasyMount Ussing Chambers Slider Inserts","description":"\u003cp\u003eP2252 EasyMount Ussing Chambers Slider Inserts\u003c\/p\u003e","products":[{"product_id":"p2252-slider-with-5-x-22mm-aperture-for-mounting-large-intestinal-tissue","title":"P2252 EasyMount Slider","description":"\u003cdiv style=\"text-align: right;\" data-mce-style=\"text-align: right;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: right;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0653\/7530\/5944\/files\/P2252_EasyMount_Ussing_Chamber_Slider_for_Rat_Intestinal_Tissues_240x240.jpg?v=1730310265\" alt=\"P2252 EasyMount Ussing Chamber Slider designed for Rat Intestinal Tissues with 5x22mm Aperture, made by Physiologic Instruments\" style=\"margin-top: 10px; margin-bottom: 16px; margin-left: 10px; float: right;\"\u003e\u003c\/div\u003e\n\u003ch3 class=\"MsoNormal\"\u003eP2252 EasyMount Slider for Mounting Intestinal Tissue\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eAPETURE SIZE: 4.775x22.02 MM | Area = 1.0 cm\u003csup\u003e2\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe P2252 EasyMount Slider has an oblong 4.775 x 22.02 mm aperture and is designed for mounting intestinal tissues with a surface area of no less than 1 cm\u003csup\u003e2\u003c\/sup\u003e and for use with EasyMount P2250 Chambers.\u003c\/p\u003e\n\u003cp\u003eOur P2252 EasyMount Slider is composed of a top clear and bottom white segment. Tissues are mounted by pressing the tissue over short, curved pins lining a matching aperture on the slider. The slider aperture has a surrounding recess to minimize edge-effect caused by light compression from mounting. The large surface area is useful when performing ion flux or drug permeability studies. The large surface area also maximizes detecting endogenous peptide secretion from the lumen or from the basolateral side.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAdvantages of the P2252 Slider:\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003eThe large surface area works with a variety of tissue specimens - rat intestine is the optimal tissue for this slider\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAperture recess area minimizes tissue edge-effect\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTissue pins aid in mounting and stretching the specimen\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTwo alignment pins provide fast and accurate closure of the two piece slider\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003chr\u003e\n\u003ch3\u003e\u003cstrong\u003eP2250 Slider Mounting Instructions:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eTo mount tissue on the P2252 EasyMount Slider and insert it into the EasyMount P2250 Ussing Chamber system, follow these steps:\u003c\/p\u003e\n\u003ch4\u003e1. \u003cstrong\u003ePreparation of the Intestional Tissue\u003c\/strong\u003e\n\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eEnsure the tissue sample is prepared to the correct size, typically at least 1 cm² to fully cover the slider’s 4.775 x 22.02 mm aperture.\u003c\/li\u003e\n\u003cli\u003eMoisten the tissue with an appropriate physiological solution to maintain its viability and flexibility.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003e2. \u003cstrong\u003eMounting the Tissue on the P2252 Slider\u003c\/strong\u003e\n\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePlace the P2252 slider on a stable surface with the aperture area accessible.\u003c\/li\u003e\n\u003cli\u003eGently position the tissue over the aperture, ensuring it covers the entire oblong opening.\u003c\/li\u003e\n\u003cli\u003eUsing tweezers or gentle manual pressure, press the tissue over the curved pins located along the aperture’s edges. These pins will secure the tissue in place by pressing it into the matching aperture.\u003c\/li\u003e\n\u003cli\u003eVerify that the tissue is evenly spread across the aperture without wrinkles or folds to maintain uniform contact with the solution.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003e3. \u003cstrong\u003eEnsuring Proper Mounting in the Slider’s Recessed Area\u003c\/strong\u003e\n\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eConfirm that the tissue fits within the recessed edge surrounding the aperture. This recess minimizes edge effects, reducing the potential for tissue distortion or leakage around the mounting area.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003e4. \u003cstrong\u003eInserting the EasyMount Slider into the P2250 Ussing Chamber System\u003c\/strong\u003e\n\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAlign the mounted P2252 slider with the P2250 Ussing Chamber. The chamber should be prepared with any necessary solutions or buffers according to the experiment's requirements.\u003c\/li\u003e\n\u003cli\u003eCarefully slide or press the P2252 into the chamber slot, ensuring it locks securely into place.\u003c\/li\u003e\n\u003cli\u003eOnce the slider is in position, check that the tissue remains centered over the aperture without slippage or gaps along the edges.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003e5. \u003cstrong\u003eFinal Adjustments and Checks\u003c\/strong\u003e\n\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eConfirm that the tissue is positioned correctly, with its luminal or basolateral side facing as required by the experiment.\u003c\/li\u003e\n\u003cli\u003eAdjust the chamber’s clamps or seals if necessary to prevent leakage and maintain an airtight seal.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAfter mounting, the system is ready for the introduction of test compounds, ion flux measurements, or drug permeability assays. If you need any assistance in these procedures, please contact us.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Physiologic Instruments","offers":[{"title":"Default Title","offer_id":43088473620696,"sku":"P2252","price":208.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0653\/7530\/5944\/products\/P2252_Ussing_Chamber_Slider_For_Rat_Intestinal_Tissues.jpg?v=1736970279"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0653\/7530\/5944\/collections\/P2250_EasyMount_Ussing_Chamber_e5d62458-1fae-4e93-b1bf-dbed0eb98919.jpg?v=1736970252","url":"https:\/\/physiologicinstruments.com\/zh\/collections\/p2252-easymount-ussing-chamber.oembed","provider":"Physiologic Instruments Inc","version":"1.0","type":"link"}