}
}
Precision & Reproducibility
Tight sealing and minimal edge damage—even for delicate tissues such as frog skin and curved epithelia.
Camere di Ussing | Slider per colture cellulari delle camere di Ussing
Physiologic Instruments ha sviluppato un'ampia gamma di supporti per colture cellulari e di tessuti, che chiamiamo cursori perché vengono utilizzati per far scorrere il campione di tessuto in posizione tra le due metà della camera di Ussing EasyMount. Le caratteristiche di questi cursori dipendono dalle caratteristiche del campione. Per gli inserti per colture cellulari, il cursore è progettato come un unico pezzo che crea una tenuta stagna tra le due metà della camera e con l'inserto di coltura, in modo che il monostrato cellulare diventi la barriera che separa i fluidi in ciascuna metà della camera.
Attualmente offriamo cursori progettati per i 5 inserti per colture più diffusi, prodotti da produttori come Corning-CoStar, Nunc e Millipore. I tessuti epiteliali, invece, provengono da un'ampia varietà di fonti e presentano diversi vincoli di dimensioni e forma. Per questo motivo, abbiamo progettato cursori specifici per i tessuti, in base alle esigenze dei nostri clienti. Le aperture su molti cursori sono di forma circolare e variano in dimensioni da 0,005 cm² (ad esempio, intestino di Drosophila o trachea di topo neonato) a 1,26 cm² (ad esempio, intestino bovino). Altri cursori presentano aperture oblunghe perché alcuni tessuti, come l'intestino di topo, sono semplicemente più lunghi che larghi.
I cursori per tessuti sono disponibili con o senza perni per tendere e trattenere il tessuto durante il processo di montaggio. Sono generalmente costituiti da cursori "in due pezzi", in cui il tessuto forma la guarnizione tra le due metà del cursore, creando un sandwich di tessuto che si inserisce tra le due metà della camera. Per tessuti "compatti" ad alta resistenza, come la pelle di rana, i cursori presentano una leggera rientranza per evitare lo schiacciamento delle cellule epiteliali lungo il perimetro dell'apertura, al fine di prevenire danni ai bordi. Per tessuti come l'epitelio o l'endotelio corneale, il cursore è progettato per adattarsi alla curvatura naturale del campione.
Physiologic Instruments Ussing Chambers
Explore your solution for ion transport, barrier integrity, and solute flux across epithelial tissues and cell monolayers—optimized for precise transepithelial resistance (TER) and short-circuit current (Isc) measurements.
Ussing Chambers Key Specifications
| Product | Application | Key Features |
|---|---|---|
| P2250 EasyMount Ussing Chamber | Rat, rabbit, guinea pig intestinal tissues | Custom slider aperture (5 × 22 mm, ~1.0 cm²); half-chamber volume 2–8 mL |
| P2300 EasyMount Ussing Chamber | Cell culture inserts, animal tissues | Versatile for monolayers and tissues; half-chamber volume 2–7 mL |
| P2400 Low-Volume Ussing Chamber | Drug solution assays, flux studies | Minimal reagent use (1–5 mL); ideal for expensive compounds |
| Chamber Sliders & Inserts | Tissue mounting and sealing | Single- or two-piece sliders; apertures ~0.005 → 1.26 cm²; curved options for non-flat tissues |
Each Ussing chamber pairs with sliders (often called Ussing chamber sliders) to mount tissues or inserts securely and maintain leak-free seals between upper and lower halves.
Why Choose Our Ussing Chambers?
Flexible Volumes & Configurations
From low-volume chambers (ideal for costly reagents) to systems for standard tissue sizes.
Custom Slider Designs
Circular/oblong apertures from ~0.005 to 1.26 cm²; curved variants for non-flat tissues.
Insert Compatibility
Integrates with popular cell culture inserts for seamless Ussing chamber workflows.
Durable, Lab-Ready
Materials selected for frequent use, sterilization, and compatibility with electrode sets and data acquisition.
Best Practices & Applications
- Tissue Preparation — Preserve margins and interfaces to prevent leaks.
- Electrode Calibration — Regularly calibrate voltage/current electrodes to avoid drift.
- Temperature & Buffers — Stabilize temperature, gas mix (O2/CO2), and pH.
- Data Quality — Monitor baseline resistance and Isc stability over time.
- Flux & Drug Studies — Prefer low-volume chambers with radiolabeled/fluorescent tracers.
These practices help ensure robust, reproducible data in epithelial transport and barrier research.
Frequently Asked Questions About Ussing Chambers
What are Ussing chambers used for?
Answer: Ussing chambers are specialized electrophysiology tools used to study ion transport, epithelial barrier integrity, and solute flux across tissues or cell monolayers. They allow measurement of transepithelial resistance (TER) and short-circuit current (Isc) under tightly controlled conditions.
Which Ussing chamber should I choose?
Answer: Choose the Ussing chamber that matches your experimental needs: the P2250 is optimized for rat, rabbit, and guinea pig intestinal tissues, the P2300 is versatile for both cell culture inserts and animal tissues, and the P2400 is a low-volume EasyMount chamber ideal for assays involving costly reagents or tracer studies.
Do you support custom sliders and apertures?
Answer: Yes. We offer a full range of single- or two-piece sliders with circular or oblong apertures ranging from ~0.005 cm² to 1.26 cm². Curved slider variants are also available to accommodate non-flat or corrugated tissues.
Are your chambers compatible with standard inserts?
Answer: Yes. Our Ussing chamber sliders integrate seamlessly with widely used inserts such as Corning Costar Snapwell and Transwell, as well as Millipore Millicell, simplifying tissue mounting and maintaining leak-free seals.
How do Ussing chambers work?
Answer: Ussing chambers work by clamping voltage and current across epithelial tissues or cell monolayers mounted between two fluid-filled half-chambers. This allows precise measurement of transepithelial ion transport, barrier properties, and solute flux under controlled temperature, gas composition, and buffer conditions.
What does 'EasyMount' mean in your Ussing chambers?
Answer: All of our Ussing chambers are branded EasyMount, referring to their design that slides directly into a heated chamber stand with support arms and a thumb screw for secure, leak-free sealing. The EasyMount design simplifies assembly, reduces setup time, and ensures reproducible results in both standard and low-volume configurations.
Resources and Support
Ussing Chamber Sliders & Inserts — full range of aperture sizes and geometries.
Voltage/Current Clamps — precise control for TER and Isc studies.
Acquire & Analyze — data acquisition and analysis tools.
Book a call — discuss applications and experimental design.