➡️ pH EpiStat Brochure - Download

pH EpiStat Automatic Titator

The pH EpiStat is a new device that works together with any of Physiologic Instruments Ussing systems – any model, any vintage up to 8 channels. The unit’s small footprint (30.5 cm x 13.3 cm x 25.5cm HxWxD) enables it to easily fit on the benchtop next to one of our  EasyMount Ussing Chamber systems. The unit is multichannel, allowing measurements from 1-8 channels almost simultaneously. The accompanying software, Acquire & Analyze Version 3.0, controls both the pH EpiStat and our voltage/current clamp instruments to enable concurrent measurements of bioelectric properties and acid or base flux across the epithelium.  

pH Stat Methodology

Measurement of acid/base transport across epithelial cells is similar to measuring the short-circuit current except that the pH, rather than voltage, is held constant. To accomplish this the pH of an unbuffered solution on one side of the epithelium is monitored while precise amounts of titrant (acid or base) are added to keep the measured pH constant. By knowing the exact volume of acid or base addition, time of addition and concentration of titrant required to “clamp” the pH, the software can calculate the rate of acid or base transport across the epithelium.

The pH EpiStat Model pH8 was engineered to accurately measure pH to within +/-0.002 pH units and inject precise volumes of titrant of less than 50nL and is controlled by the latest version of Acquire & Analyze (version 3). Together, titrant additions required to hold the pH constant are accurately tracked and the rate of acid/base transport across the epithelium is calculated.

Requires: Acquire & Analyze 3

High Content Transport Studies

Epithelial pH homeostasis is an important area in research. In virtually every tissue and organ system epithelial cells have been demonstrated to play a role in pH regulation. This regulation generally involves multiple mechanisms including active, passive and exchange transport systems. The pH EpiStat can measure the rate of acid/base transport. However, when combined with our voltage/current clamp device and the short-circuit technique, experiments can be designed to delineate, for example,  between the movement of an anion such as chloride compared to bicarbonate, or cation such as sodium movement compared to hydrogen ions. In research, time is money. By enabling simultaneous measurement of both bioelectric data and pH, the system becomes a true high-content tool, providing richer datasets and greater efficiency for the end-user.

The pH EpiStat comes well equipped with an automated titrator and syringe, customized pH electrodes, titrant line tubing, titrant container, specialized chamber caps, dual air valves, calibrator bath, calibration buffer, and Acquire and Analyze Version 3 Software along with two days of installation and training.

Included system components are:

ncluded System Components

Qty	Description
1	pH EpiStat revA pH measuring & titrating device with power cord
8	pH-E1 custom fast response pH electrodes (PHYSI-001)
8	P2062 dual gas air valves (replaces P2060 installed on stand)
8	P2300 pHCap (positions pH electrodes & titrant tubes)
8	P2300 Cap (for the other side of chamber)
8	PT.02 titrant lines
2	PT.04 prime/waste lines
10	Flangeless fittings for tubing (attached to titrant & waste lines)
1	WB660 temperature-controlled calibration reservoir
1	WBEH pH electrode holder for calibration with ground wire
3	60 ml each of pH 6.0, 7.0, and 8.0 calibration buffer
4	Calibration beakers
1	Titrant bottle
1	Waste bottle
8	Priming stands and holder
1	GWB1 gas washing bottle and tubing
1	100 µl glass bubble-free syringe
1	Reusable calibration beaker holder
8	pH electrode storage vials with lids
8	pH titrant priming vials
1	USB cable
1	pH electrode leads cable management sleeve
3	Day installation and training (travel expenses billed separately)

Note: Requires Acquire & Analyze (A&A) Version 3.

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Complete pH-Clamp Workflow

A fully integrated workflow from tissue to data.
The EpiStat module works with Acquire & Analyze to automate pH control, titrant delivery, and acid/base flux calculations. Set your target pH and let the system maintain it with precision throughout the experiment.

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Designed for Epithelial Transport Research

Built for Ussing chamber studies involving mucosal physiology, GI epithelium, pulmonary tissue, ion exchange pathways, and transporter-specific mechanisms such as NHE, CFTR, DRA, and H+/K+ ATPase.

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Multichannel Throughput

Run multiple tissues at once with 1–8 independent titration channels — each with its own pH clamp, titrant path, and data stream. Perfect for repeated dose-response experiments, inhibitor comparisons, and multi-tissue studies.

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Automated pH Control With Micro-Volume Precision

• Real-time titrant delivery

• Nanoliter-scale precision

• Continuous pH feedback

• Fast settling with minimal oscillation

• Stable baselines for long experiments

This eliminates manual corrections and improves reproducibility across runs.

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Acquire & Analyze Integration

Acquire & Analyze captures electrode outputs, adjusts titrant delivery, logs all activity, and exports ready-to-use data tables. pH, current, voltage, resistance, and flux data remain synchronized across all channels.

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Built for High-Accuracy Physiology Work

Stable, low-noise pH inputs and durable channel hardware enable consistent measurements across extended experiments. Each unit is calibrated and verified before shipment for accurate titration control.

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Compatible With Our Ussing Chamber Platforms

Works seamlessly with:

• EasyMount chambers
  

• Inserts, sliders, Snapwell-style setups

• Tissue monolayers and epithelial cultures

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Ideal Research Applications

• Acid/base secretion studies

• Intestinal proton transport

• CFTR + NHE interactions

• Gastric epithelium pH regulation

• Drug permeability and pH-dependent transport

• Airway surface liquid studies

• Nutrient absorption assays

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Key Functional Highlights

• 1–8 independent pH-clamp channels

• Automated titration with micro-volume control

• Priming, purge, aspirate, and titrate functions

• pH stability tracking

• Full experiment logging

• Integration with A&A for data export

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Included

• Calibration and verification testing before shipping

• Software setup assistance

• Guidance on integrating with existing Ussing chambers

• Documentation for pH probe setup and titrant path configuration

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Compatible Accessories

Supports:

• pH electrodes designed for fast response

• Titrant reservoirs and tubing sets

• Channel connectors and chamber adapters

• Acquire & Analyze software packages

pH EpiStat Automatic Titrator on display