Ussing Chamber Systems

Complete Ussing Chamber Systems for Cell & Tissue

Uso de sistemas de cámara

Se utiliza una cámara de Ussing para estudiar el transporte de solutos a través de una capa confluente de células. Esta capa suele ser una monocapa de células epiteliales que forman una barrera entre los entornos externo e interno del cuerpo, como los que recubren las vías respiratorias, el tracto intestinal y la vejiga, pero también podría ser una estructura de varias capas, como la piel o un cultivo celular en 3D o una capa confluente de células endoteliales como la barrera hematoencefálica en cultivo o el endotelio corneal. El transporte de soluto se puede determinar midiendo el flujo del soluto o, a veces, en el caso de solutos cargados como los iones, midiendo la corriente a través del tejido. Estos parámetros eléctricos, junto con la resistencia del tejido, sirven para evaluar el transporte de iones y la integridad del tejido.


Para realizar estas mediciones, los sistemas Ussing han sido diseñados para tener tres componentes principales; a saber, cámara Ussing, pinza de tensión/corriente y sistema de adquisición de datos.

1) La cámara de Ussing es simplemente un dispositivo que permite colocar el tejido entre dos compartimentos llenos de solución. La cámara puede estar equipada con electrodos para medir el voltaje y hacer pasar corriente a través del tejido.

2) La pinza de corriente de voltaje se utiliza para controlar el voltaje o la corriente a través del epitelio y así determinar elementos bioeléctricos como el voltaje transepitelial, la corriente de cortocircuito y la resistencia o conductancia.

3) Los resultados de las mediciones eléctricas se pueden registrar usando algo tan simple como un registrador gráfico o versiones electrónicas más nuevas de un registrador gráfico. Alternativamente, se puede recopilar mediante un sistema inteligente de adquisición de datos que actúa como un registrador gráfico pero también mide y traza la resistencia o conductancia del tejido en tiempo real, como Acquire & Analyse.

Sistemas Ussing con todas las funciones que se componen de nuestro sistema de cámara EasyMount Ussing, instrumentos de pinza de corriente de voltaje multicanal y paquete de adquisición de datos Acquire & Analyse junto con accesorios para establecer un sistema funcional casi completo. Estos sistemas están disponibles en versiones de 2, 4, 6 y 8 canales basados ​​en una de las tres cámaras Ussing diferentes.

Sistema de uso EasyMount

Ussing Chamber Systems

Our integrated Ussing chamber system platforms combine precision EasyMount chambers, multi-channel voltage/current clamps, and real-time data acquisition for high-fidelity Vte, Isc, Rte, and Gte measurements across cultured cells and tissues. Compare systems below; full included components are shown in the cards that follow.

System Comparison

System Primary Use Chamber Model Working Volume (per half) Tissue / Holders Channels Options Links
EM8-C
Cell & Tissue
General epithelial transport & barrier studies with flexible sample formats. P2300 EasyMount 2–7 mL (typ. 5 mL) Interchangeable P23xx sliders for cell inserts and a range of animal/human tissues. 2 / 4 / 6 / 8 Base, CE, High Voltage (±35 V), CE + HV Includes
Product
EM8-LV
Low Volume
Low net transport or assays requiring higher solute concentrations. P2400 EasyMount (low volume) 1.0–5.0 mL Choice of holders; supports biopsies (intestinal/nasal), mouse pup intestine, cornea; up to 0.71 cm² (Ø 9.5 mm). 2 / 4 / 6 / 8 Base, CE, High Voltage (±35 V), CE + HV Includes
Product
EM8-R
Rat Tissue
Specialized for rat intestinal tissue mounting and analysis. P2250 EasyMount (rat) 2.0–7.0 mL (typ. 5 mL) P2252 slider with curved pins; 5 × 22 mm oblong aperture → 1.0 cm² tissue area. 2 / 4 / 6 / 8 Base, CE, High Voltage (±35 V), CE + HV Includes
Product
Notes & Best Practices:
  • Pair each Ussing chamber system with Ag/AgCl electrodes; validate Isc/Rte scaling in Acquire & Analyze before runs.
  • Match holder/aperture to tissue geometry; for low-volume assays, confirm target bath concentrations.
  • Use gas lift (air/95–5) for mixing/oxygenation; keep water-bath regulation stable across channels.

Included Components (by System)

EM8-C — Included Components

  • VCC MC8-8S voltage/current clamp with OptiGain
  • DM-MC6 Input Modules with Dummy Membrane (×8)
  • Acquire and Analyze Data Acquisition package with DI-720P
  • EM-CSYS-8 Chamber System
  • P2300 Ussing Chambers (×8) & P2300 Chamber Caps (×8)
  • P2020-S Electrode Sets (×8) & P2024-40 Electrode Lead Sets (×8)
  • P2060 Air Valve Sets (×8)
  • FN15 Filling Needles (×16)
  • GWB1 Gas Washing Bottle (1 L)
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EM8-LV — Included Components

  • VCC MC8-8S voltage/current clamp with OptiGain
  • DM-MC6 Input Modules (×8)
  • Acquire & Analyze Data Acquisition package with DI-720P
  • P2400 Low-Volume Ussing Chambers (×8) & caps (×8)
  • P2020-S Electrode Sets (×8) & P2024-40 Electrode Lead Sets (×8)
  • P2060 Air Valve Sets (×8)
  • FN15 Filling Needles (×16)
  • GWB1 Gas Washing Bottle (1 L)
  • P2400 series sliders are purchased separately; choose holder per chamber.
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EM8-R — Included Components

  • VCC MC8-8S voltage/current clamp with OptiGain
  • DM-MC6 Input Modules (×8)
  • Acquire & Analyze Data Acquisition package with DI-720P
  • P2250 Rat Tissue Ussing Chambers (×8) & caps (×8)
  • P2020-S Electrode Sets (×8) & P2024-40 Electrode Lead Sets (×8)
  • P2060 Air Valve Sets (×8)
  • FN15 Filling Needles (×16)
  • GWB1 Gas Washing Bottle (1 L)
  • P2252 sliders (curved pins, 5×22 mm, 1.0 cm² tissue area) — recommend ordering extra
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Why Labs Choose Our Ussing Chamber Systems

Precision & Reproducibility

EasyMount mechanics and OptiGain clamp performance deliver stable Vte/Isc baselines, fast settling, and repeatable Rte/Gte calculations.

Throughput Without Compromise

2–8 channel configurations support simultaneous studies while maintaining uniform temperature control, gas lift mixing, and electrode performance.

Modular & Application-Ready

Swap sliders/holders to match tissue geometry and study design—from cell inserts to low-volume biopsies to dedicated rat tissue prep.

EasyMount Ussing Chamber Systems Features

  • EasyMount, leak-free setup: chamber slides into the heated stand; arms and a thumb screw secure it without damaging tissue edges.
  • Low reagent use when needed: P2400 low-volume option minimizes mL per half-chamber for expensive compounds.
  • Insert & tissue compatibility: supports Snapwell/Transwell/Millicell inserts and native tissues.
  • Throughput that scales: 2, 4, 6, or 8 channels with VCC MC2/MC6/MC8 for parallel experiments.
  • Training & support: protocols, calibration guidance, and application help.

Key Measurements and Applications

Measurements: TER/TEER (barrier integrity), Isc (active transport at ~0 mV), I–V curves, and solute flux/permeability (radiolabeled/fluorescent tracers).

Applications: GI, airway, renal, and skin epithelia; CFTR/ENaC studies; drug permeability and transporter function; inflammation and tight-junction research.

Resources & Support

Chamber Sliders — choose apertures/holders for each Ussing chamber system.

Voltage/Current Clamps — multi-channel control for Vte, Isc, and Rte protocols.

Acquire & Analyze — real-time Rte/Gte, stimulus protocols, and export.

Electrodes & Accessories — Ag/AgCl electrodes, leads, air valves, gas wash bottles.

Talk to an applications specialist — get help matching a system to your assay.

Frequently Asked Questions

Which Ussing chamber system should I choose?

EM8-C for versatile cell/tissue work, EM8-LV for low-volume or high-concentration assays, and EM8-R for rat intestinal tissue workflows.

Can I measure tissue resistance in real time?

Yes. With Acquire & Analyze, Rte and Gte are computed and plotted live alongside Vte and Isc.

Do I need different sliders/holders for different tissues?

Yes. Select sliders that match your tissue geometry and aperture target. For P2400 systems, sliders are purchased separately per chamber.

What channel count do I need?

2–4 channels suit smaller labs and method development; 6–8 channels support higher throughput and parallel comparisons.