EasyMount Ussing System for Cell & Tissue

The EasyMount Ussing Chamber System, based on the P2300 chamber, uses the P23xx series slider to mount both cell culture inserts and a wide variety of tissues.  Working chamber volumes are 2-7 mL.

The EM8-C Cell & Tissue Ussing System Includes:

(1) VCC MC8-8S voltage/current clamp

(1) optiGain for switching current gain and fluid resistance ranges

(8) DM_MC6 Input Modules with Dummy Membrane

(1) EM-CSYS-8 Chamber System

(1) 8-Channel EasyMount Chamber Stand

(8) P2300 Chambers

(8) P2020-S Electrode sets

(8) P2024-40 Electrode Lead Sets

(8) P2060 Air Valve Sets

(8) P2300-caps (chamber caps)

(2) P2020-S Extra Electrode sets

(3) P2023-100 electrode tips (100/bag)

(16) FN15 Filling needles

(1) GWB1 Gas Washing Bottle (1 L)

(1) TK-7 Tubing and setup kit

(1) Acquire & Analyze Data Acquisition package with DI-720P

A/D Converter System does not include sliders as these are specific to your experiments;  Up close with our Ussing Chamber & Stand Anodized Aluminum Heat Block with Cradles Precision Acrylic Ussing Chamber with Electrodes Chamber Caps & Adjustable Air Valves Ideal gas lift circulation Used to measure the net ion transport taking place across the epithelium A voltage is generated across the epithelium as a consequence of ion selective channels in the membranes and active transport. This voltage is sensed by the black Ag/AgCl electrodes and measured by the voltage clamp instrument. Current may be passed by the clamp instrument across the epithelium via the white electrodes. When this current is sufficient to exactly cancel the voltage generated by the epithelium, the epithelium is “short-circuited” and the current necessary to do this is the short-circuit current (Isc). When the solutions in the chamber compartments are the same, the Isc is a measure of the net active transport of ions across the epithelium. VCC MC8-8S voltage current clamp optiGain for switching current gain and fluid resistance ranges Input Modules (DM MC6) & Dummy Membrane8-Channels for each chamber Pulse Generator Master Control Acquire & Analyze Data Acquisition Specifically for measuring bioelectrics from small pieces of epithelial tissue or cells Data Acquisition Hardware Interface cables ACQUIRE and ANALYZE software runs on Windows® to measure current, voltage, conductance and resistance from one to eight tissues simultaneously.