P2300 EasyMount Ussing System for Cell & Tissue

P2300 EasyMount Ussing System for Cell & Tissue

The P2300 EasyMount Ussing System for Cell & Tissue is designed for advanced studies of ion transport, barrier function, and electrophysiological properties across epithelial and cell culture samples. By employing interchangeable P23xx series sliders, this system accommodates a range of tissue types, from cultured cell inserts to specialized animal and human tissues, making it highly versatile for various research applications. Its working volume range of 2–7 mL (with an optimal volume of 5 mL) provides flexibility for maintaining physiological conditions, which is essential in accurately modeling in vivo environments. This flexibility allows researchers to control luminal and basolateral environments, supporting high-precision studies on epithelial tissues and aiding in characterizing their transport properties.

With integrated components like the VCC MC8-8S voltage/current clamp and the Acquire & Analyze Data Acquisition package, the P2300 system ensures high-resolution, stable measurements across experiments. The system’s modularity allows for simultaneous multi-sample analysis, supported by the DM-MC6 Input Modules, making it ideal for laboratories studying barrier integrity, ion channel function, or responses to pharmacological treatments. The P2300’s configuration promotes reproducibility and accuracy, key for studies requiring quantitative analysis of epithelial function, and positions it as an indispensable tool for researchers in physiology, pharmacology, and cellular biology.

The EM8-C Cell & Tissue Ussing System Includes:

The P2300 EasyMount Ussing Chamber System is a comprehensive, adaptable setup designed for in-depth studies of ion transport and barrier function in epithelial and cultured cell samples. This system uses the versatile P23xx series of EasyMount sliders, allowing researchers to mount a variety of tissue samples and cell culture inserts with ease. With a working chamber volume of 2–7 mL, and a typical working volume of 5 mL, the P2300 system supports a wide range of sample types and experimental conditions, ensuring precision and stability across studies.

Included in the EM8-C Cell & Tissue Ussing System are essential components for reliable and reproducible measurements. The VCC MC8-8S voltage/current clamp with optiGain provides sensitive and accurate control, essential for studies involving epithelial resistance and ion transport measurements. The system is complemented by the Acquire & Analyze Data Acquisition package with DI-720P, streamlining data collection and processing. The setup includes eight DM-MC6 Input Modules with Dummy Membrane, supporting simultaneous multi-sample analysis.

Chamber parts include eight P2300 chambers, equipped with P2020-S electrode sets and P2024-40 electrode leads for reliable, high-quality connectivity. Accessories like the EM-CSYS-8 Chamber System, FN15 filling needles, and GWB1 gas washing bottle ensure the system is ready for immediate experimental use. Additionally, the P2300-Caps and P2060 air valve sets allow fine control of gas flow and environment stability, essential for maintaining physiological conditions. This complete, fully-integrated system makes the P2300 EasyMount Ussing Chamber System ideal for cutting-edge epithelial and cell culture research.

A/D Converter System does not include sliders as these are specific to your experiments;  Up close with our Ussing Chamber & Stand Anodized Aluminum Heat Block with Cradles Precision Acrylic Ussing Chamber with Electrodes Chamber Caps & Adjustable Air Valves Ideal gas lift circulation Used to measure the net ion transport taking place across the epithelium

A voltage is generated across the epithelium as a consequence of ion selective channels in the membranes and active transport. This voltage is sensed by the black Ag/AgCl electrodes and measured by the voltage clamp instrument. Current may be passed by the clamp instrument across the epithelium via the white electrodes. When this current is sufficient to exactly cancel the voltage generated by the epithelium, the epithelium is “short-circuited” and the current necessary to do this is the short-circuit current (Isc).

When the solutions in the chamber compartments are the same, the Isc is a measure of the net active transport of ions across the epithelium. VCC MC8-8S voltage current clamp optiGain for switching current gain and fluid resistance ranges Input Modules (DM MC6) & Dummy Membrane8-Channels for each chamber Pulse Generator Master Control Acquire & Analyze Data Acquisition Specifically for measuring bioelectrics from small pieces of epithelial tissue or cells Data Acquisition Hardware Interface cables ACQUIRE and ANALYZE software runs on Windows® to measure current, voltage, conductance and resistance from one to eight tissues simultaneously.

EasyMount Ussing System options:

EM8-C - standard system

EM8-C-CE - standard system with EU CE certification

EM8-C-HV - high voltage (+/- 35 V output) system

EM8-C-CE-HV - high voltage (+/- 35 V output) system with EU CE certification

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Product References:    Scholar Papers written using our EasyMount Ussing Systems  

	Published: 18 June 2024 Cinnamic Acid, Perillic Acid, and Tryptophan Metabolites Differentially Regulate Ion Transport and Serotonin Metabolism and Signaling in the Mouse Ileum In Vitro
	Published: 29 February 2024 Reduced surface pH and upregulated AE2 anion exchange in SLC26A3-deleted polarized intestinal epithelial cells
	Published: 21 May 2024 - Thematic Poster Session / San Diego Convention Center Comparison of the Effectiveness of Angiotensin Receptor Blockers in Reversing TGF-β1-Induced CFTR and Mucociliary Dysfunction in Cystic Fibrosis Airway