P2302M EasyMount Slider

P2302M EasyMount Slider for Millipore Millicell Culture Insert

APETURE SIZE: 8.75MM | Membrane Area = 0.60 cm², accepts Millicell culture inserts

The P2302M EasyMount Slider is engineered for precise compatibility with Millipore Millicell Culture Inserts, providing an 8.75 mm diameter aperture that securely accommodates Millicell inserts used in 24-well plates. Designed to facilitate studies on cellular transport, permeability, and barrier function, the P2302M slider exposes a defined membrane area of 0.60 cm², enabling controlled and consistent sample interface. This slider's design ensures optimal stability and alignment, preserving the structural integrity of the membrane while providing a reliable framework for accurate measurement and data collection.

Ideal for use in Ussing Chamber systems, the P2302M EasyMount Slider supports high-quality studies by maintaining precise positioning of the Millicell culture insert within the chamber. This configuration allows researchers to perform detailed investigations into cellular and molecular transport processes, as well as barrier function assays, under various experimental conditions. The P2302M’s secure fit and robust construction make it an indispensable tool in cell culture and physiological research, offering reproducible results and streamlined setup for demanding experimental protocols

P2302M EasyMount Ussing Chamber Slider Instructions

To effectively use the P2302M EasyMount Slider in an experiment involving the Millipore Millicell Culture Insert and the P2300 Ussing Chamber, follow these detailed instructions on mounting, setup, and conducting the experiment.

1. Preparing the Millicell Culture Insert

• Cell Seeding and Preparation: Seed the Millipore Millicell Culture Insert with your cell type of interest, and culture the cells according to your experimental requirements. Ensure that the cell layer has reached the desired level of confluence or differentiation, typically forming a barrier for transport studies.
• Rinse and Hydrate: Before mounting, rinse the insert with physiological buffer (such as Ringer’s solution) to ensure that the membrane is hydrated and equilibrated, which can help stabilize the tissue for experimental conditions.

2. Mounting the Insert in the P2302M Slider

• Positioning the Insert: Place the Millicell Culture Insert into the 8.75 mm aperture of the P2302M EasyMount Slider, ensuring the membrane area aligns with the 0.60 cm² aperture. The insert should fit snugly within the slider to prevent movement and maintain a secure seal.
• Securing the Insert: Confirm that the culture insert is stable within the P2302M slider, with no gaps or irregularities around the edges. This positioning allows for a tight seal, which is critical to ensuring consistent exposure on both sides of the membrane for transport measurements.

3. Setting Up in the P2300 Ussing Chamber

• Prepare the Chamber Solutions: Fill each compartment of the P2300 Ussing Chamber with the desired physiological solution. This could be identical solutions on both sides for baseline measurements or different solutions to create gradients, depending on the experiment's purpose.
• Inserting the Slider: Place the P2302M slider, with the Millicell insert, into the P2300 Ussing Chamber. Ensure the insert is properly centered and aligned to maintain a leak-free setup. The slider should hold the insert in a way that each side of the membrane faces a different chamber compartment.
• Placing Electrodes and Sensors: Position voltage and current electrodes in each chamber compartment, as specified by the P2300 Ussing Chamber setup, for measuring electrical potential and transport properties across the cell layer.

4. Conducting the Experiment

• Baseline Measurements: Begin by recording baseline parameters such as transepithelial resistance (TER) and potential difference (PD) to confirm the integrity of the cellular barrier and establish initial conditions.
• Introducing Experimental Conditions: Add experimental compounds or treatments (e.g., drugs, ion solutions) to the appropriate side(s) of the chamber to study their effects on cellular transport or permeability across the membrane.
• Monitoring and Data Collection: Continuously monitor and record parameters of interest (e.g., TER, short-circuit current, concentration gradients) over time to assess the cell layer’s transport characteristics in response to the experimental conditions.

5. Completing the Experiment and Cleaning the Equipment

• Data Analysis: Save and analyze the collected data to evaluate transport and barrier properties of the cell layer within the Millicell insert.
• Disassembling and Cleaning: Carefully remove the P2302M slider from the Ussing chamber, dispose of or properly clean the Millicell insert if it will be reused. Clean the P2302M slider, Ussing chamber components, and any electrodes with distilled water, followed by a suitable cleaning solution, to maintain their integrity for future experiments.

Using the P2302M EasyMount Slider with the Millicell insert in the P2300 Ussing Chamber provides a consistent and reproducible platform for investigating cell culture transport and permeability, ensuring that both experimental setup and data collection are reliable and accurate. If you need any assistance, please contact us.