Ussing Chambers

P2300 EasyMount Ussing Chambers

Physiologic Instruments has developed a wide selection of tissue and cell culture holders that we call "sliders" because they are used to "slider" the tissue sample into place between the two chamber halves of the EasyMount Ussing chamber. The features of these sliders depend on the characteristics of the sample. For cell culture inserts the slider is designed as a single piece that makes a water tight seal between the chamber halves as well as with the culture insert so that the cell monolayer becomes the barrier separating the fluids in each chamber half.

We currently offer sliders designed for the 5 most popular culture inserts from manufacturers such as Corning-CoStar, Nunc and Millipore. Epithelial tissues, on the other hand, derive from a wide variety of sources and have a variety of size and shape constraints. For this reason we have designed specific sliders for tissues as required by our customers. The apertures on many sliders are circular in shape and range in size from 0.005 cm2­­(e.g., drosophila intestine or newborn mouse trachea) to 1.26 cm2 (e.g., bovine intestine). Other sliders have oblong apertures because some tissues, such as mouse intestine, are simply longer than they are wide.

Tissue sliders come with or without pins for stretching and holding the tissue during the mounting process. They are generally a “two-piece” slider with the tissue forming the seal between the slider halves creating a tissue sandwich that then inserts between the chamber halves. For high resistance “tight” tissues such as frog skin, the sliders have a slight recess to prevent crushing the epithelial cells along the aperture perimeter in order to prevent edge damage. For tissues such as corneal epithelium or endothelium the slider is designed to handle the natural curvature of the specimen.
  • EasyMount Ussing Chamber P2250 - Physiologic Instruments
    P2250 EasyMount Ussing Chamber for Rat Tissues
    Designed for Rat Intestinal Tissues

    The P2250 Intestinal EasyMount Ussing Chamber is a specialized research tool for studying transport and barrier functions in intestinal tissues, particularly from species like rats, guinea pigs, and rabbits. It features a custom EasyMount Ussing Chamber Slider (P2252) with a 5 x 22 mm oblong aperture, providing a 1.0 cm² area ideal for mounting intestinal samples securely and precisely. With a flexible usable half-chamber volume range of 2.0 to 8.0 ml, the P2250 is adaptable to various experimental protocols. Its design prioritizes durability, ease of use, and reliable data collection, making it a valuable instrument for gastrointestinal research.


    ⦿ To be used with EasyMount P2252 Chamber Slider Insert

    $637.00
    $637.00
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  • EasyMount Ussing Chamber P2300 - Physiologic Instruments
    P2300 EasyMount Chamber
    Designed for Animal Tissues & Cell Cultures

    The P2300 EasyMount Ussing Chamber is designed as a versatile and reliable instrument for experimental research focused on transport and barrier functions of biological tissues and cell monolayers. It incorporates an intuitive design that accommodates a broad range of sample types, from native animal tissues to cultured cell layers on permeable supports, with straightforward integration into both standard and customized laboratory workflows. Equipped with precision-engineered EasyMount sliders, the P2300 simplifies the setup and alignment of samples, minimizing handling time and enhancing reproducibility, crucial for consistent results across experiments.

    The chamber’s flexibility in half-chamber volume (2.0–8.0 ml) allows researchers to tailor the setup to specific experimental needs, optimizing resources while maintaining accuracy. With durable construction materials suited for frequent use and compatibility with various physiological measurement devices, the P2300 is not only adaptable but also built to last, supporting the rigorous demands of high-quality research in physiology, pharmacology, and related fields.


    ⦿ To be used with P2300 EasyMount Chamber Slider Inserts

    $635.38
    $635.38
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  • EasyMount Ussing Chamber P2400 - Physiologic Instruments
    P2400 EasyMount Low Volume Ussing Chamber
    Designed for Drug Solutions & FLUX Measurements

    The P2400 Low-Volume EasyMount Ussing Chamber is tailored for studies involving animal tissues, specifically designed to optimize experiments requiring minimal reagent volumes. With a usable half-chamber volume of 1.0 to 5.0 ml, it is ideal for research using expensive drug solutions or FLUX measurements. The P2400 uses custom EasyMount sliders for precise and secure tissue mounting, accommodating various tissue types for versatile applications. Sold in pairs for efficient experimental setups, the P2400 offers durability, ease of use, and cost-saving benefits, making it a valuable tool for precise, low-volume physiological studies.

    ⦿ To be used with P2400 EasyMount Low-Volume Slider Inserts

    $659.00
    $659.00
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