P2302N EasyMount Slider

P2302N EasyMount Slider for Nunc/Anapore Culture Insert

APETURE SIZE: 8.0MM | Area = 0.5 cm². Dia = 10mm

Optimize your transport and electrophysiological studies with the P2302N EasyMount Slider. Featuring an advanced Nunc/Anapore polycarbonate culture insert with an 8 mm tissue-culture-treated membrane supported by a removable ring, this device ensures stable, adaptable positioning in EasyMount ussing chambers. The P2302N EasyMount Slider is an essential tool for enhancing research efficiency and precision.

P2302N Use with Nunc Polycarbonate Membrane Insert

To use a Nunc Polycarbonate Membrane Insert with a Physiologic Instruments EasyMount P2302N Ussing Chamber Slider, follow these general steps:

1. Prepare the Nunc Membrane Insert: Begin by selecting the appropriate pore size (0.4, 3, or 8 microns) based on your experimental requirements. These inserts come pre-sterilized, so ensure they remain in a sterile environment before use. If needed, condition the membrane by rinsing it with a suitable buffer or medium to promote optimal cell attachment and minimize any initial diffusion imbalances.
2. Load the Insert into the EasyMount Slider: The EasyMount P2302N Slider is designed to hold culture inserts in a secure, detachable ring, which provides stable positioning within the chamber. Place the Nunc Membrane Insert in the ring, ensuring it is centered and lies flat. The ring’s design allows the insert to be positioned for either vertical or horizontal diffusion, depending on the specific needs of the experiment. For horizontal setups, simply place the slider and ring assembly in the horizontal orientation within the chamber.
3. Position the Slider in the Ussing Chamber: Once the insert is secured in the EasyMount Slider, carefully place it into the Ussing chamber apparatus. The chamber setup will create separate compartments on either side of the membrane insert, allowing you to study transport processes, permeability, or electrophysiological parameters across the cell monolayer or tissue sample cultured on the membrane. Fill each chamber side with the appropriate buffer solution or media, adjust the temperature and flow rates if needed, and begin monitoring or applying electrical current as required for your study.

This setup with the Nunc Membrane Insert in the EasyMount Slider within the Ussing chamber is ideal for various assays, such as measuring ion transport, permeability, and barrier integrity of epithelial and endothelial cell layers. The configuration ensures stability and versatility for controlled, reproducible results in diffusion or electrophysiological studies.