P2304 EasyMount Ussing Chamber Slider

P2304 EasyMount Ussing Chamber Slider for Tissues Such As Mouse Intestine

APETURE SIZE: 2.7x11.2MM | Membrane Area = 0.3 cm² | With Mounting Pins

The P2304 Ussing Chamber Slider is designed for precise physiological studies involving elongated tissue specimens, such as mouse intestine. Featuring a 2.7 x 11.2 mm aperture with a 0.3 cm² exposure area, this specialized slider provides an optimized surface for accurately assessing transport and barrier functions in extended tissue samples, allowing for reproducible and consistent results across experiments.

Compatible with the EasyMount Chamber Model P2300, the P2304 slider incorporates eight mounting pins to securely position tissue over the aperture, minimizing movement and potential mechanical stress. This stability preserves tissue integrity, ensuring reliable data collection throughout the study. The P2304 Ussing Chamber Slider is an essential tool for researchers requiring a dependable, high-precision solution for advanced physiological studies with elongated tissue samples.

Instructions on how to use the P2304 EasyMount Ussing Chamber Slider Insert

To use the P2304 Chamber Slider with the P2300 EasyMount Chamber System, follow these steps to ensure a successful setup and obtain accurate experimental results:

1. Prepare the Tissue Sample: Carefully excise the tissue specimen—such as a section of mouse intestine—ensuring it fits the 2.7 x 11.2 mm aperture of the P2304 slider. Trim the sample to cover the 0.3 cm² exposure area evenly, which is essential for consistent experimental conditions across the entire tissue area.

2. Mount the Tissue on the Slider: Position the tissue over the aperture on the P2304 slider, making sure it is centered and smooth. Secure it in place using the eight mounting pins. These pins are specifically designed to hold the tissue securely without causing mechanical stress, preserving sample integrity for reliable data collection.

3. Insert the Slider into the EasyMount Ussing Chamber: Once the tissue is properly mounted, carefully place the P2304 slider into the EasyMount Chamber P2300. Ensure that the slider aligns precisely within the chamber to create a tight seal, which is important to prevent buffer leakage and guarantee uniform exposure of the tissue to buffer solutions on both sides.

4. Prepare the Ussing Chamber for the Experiment: Fill the reservoirs of the EasyMount Ussing Chamber with the appropriate physiological buffers for your study. Adjust conditions, such as pH and temperature, based on experimental protocols. Ensure that buffer flow or stirring is consistent if required, as this can affect transport or barrier function measurements.

5. Initiate Measurements: With the setup complete, start your experimental measurements. Typical electrophysiological assessments, like transepithelial potential difference (TEPD) or short-circuit current (Isc), can be performed to analyze transport properties or barrier functions. The P2304’s elongated aperture is particularly suited for such studies in tissues like mouse intestine.

6. Post-Experiment Procedures: After the experiment, carefully remove the slider from the chamber. Release the tissue from the pins and proceed with any further analyses or disposal as required. Clean the P2304 slider and P2300 chamber components to ensure they remain in optimal condition for future experiments.

Following these steps ensures that the P2304 Chamber Slider and P2300 EasyMount Chamber System are used effectively to produce high-quality, reproducible data in physiological studies involving elongated tissue specimens. If you need any assistance with using the P2304 slider please contact us.