P2304S EasyMount Ussing Chamber Slider

P2304S EasyMount Ussing Chamber Slider with Silver Electrodes for Tissues Such As Mouse Intestine

APETURE SIZE: 2.7x11.2MM | Area = 0.3 cm² | Silver Electrodes for Stimulating Tissue

The P2304S Ussing Chamber Slider is a specialized component designed for detailed physiological studies on elongated tissue specimens, such as sections of mouse intestine. It features a 2.7 x 11.2 mm aperture, providing an optimal 0.3 cm² exposure area, which is ideal for accurate assessment of transport and barrier functions in extended tissues. This carefully sized aperture ensures that researchers can focus on specific tissue regions, delivering reliable and reproducible results for various experimental needs.

In addition to its elongated design, the P2304S Ussing chamber slider includes integrated silver electrodes for transmural electric field stimulation, making it suitable for studies requiring controlled electrical stimulation across tissue layers. This unique feature allows researchers to examine tissue responses under electric field conditions, expanding the experimental capabilities of the EasyMount Ussing chamber P2300. The P2304S Ussing chamber slider is a comprehensive solution for researchers seeking precise control and enhanced functionality in studies of small, elongated tissue samples.

P2304S Ussing Chamber Slider Instruction

To use the P2304S slider in the P2300 EasyMount ussing chamber system, follow these detailed steps to ensure optimal setup and reliable experimental results:

1. Prepare the Tissue Sample: Carefully excise the tissue specimen, such as mouse intestine, to align with the 2.7 x 11.2 mm aperture of the P2304S slider. Trim and position the tissue to fully cover the 0.3 cm² exposure area, ensuring uniform thickness and smooth placement over the aperture.

2. Mount the Tissue on the Slider: Place the tissue over the aperture on the P2304S ussing chamber slider. Secure it in position using the eight mounting pins, which are specifically designed to hold the sample firmly without inducing mechanical stress or damage. This setup maintains sample integrity, which is essential for achieving consistent results.

3. Set Up the Slider in the EasyMount Ussing Chamber: Once the tissue is mounted, insert the P2304S slider into the EasyMount Ussing chamber P2300. Make sure that the slider is properly aligned to create a tight seal within the chamber, preventing buffer leakage and ensuring an even distribution of the buffer solutions on both sides of the tissue.

4. Prepare for Electric Field Stimulation: The P2304S slider includes integrated silver electrodes for transmural electric field stimulation. Connect the electrodes to the appropriate stimulation or measurement equipment. Verify all connections and settings according to your protocol to ensure the electric field is applied consistently across the tissue during the experiment.

5. Conduct the Experiment: Fill the chamber reservoirs with the necessary physiological buffers, maintaining consistent pH and temperature based on experimental requirements. Initiate electric field stimulation and electrophysiological measurements as needed, such as transepithelial potential difference (TEPD) or short-circuit current (Isc), to assess transport properties or barrier function under the influence of electric field modulation.

6. Post-Experiment Handling: After completing the experiment, carefully disassemble the setup by first disconnecting the electrodes and then removing the slider from the chamber. Gently release the tissue from the mounting pins and proceed with any further analyses or sample disposal. Clean and maintain the P2304S slider and chamber components to ensure optimal performance in future experiments.

By following these steps, you can effectively use the P2304S slider within the EasyMount Chamber System to achieve controlled electric field stimulation and high-quality results in studies involving elongated tissue samples. If you need any assistance on using our P2304S slider, please contact us.