P2305 EasyMount Slider

P2305 EasyMount Slider for Rat, Rabbit, or Guinea Pig Intestine

APETURE SIZE: 4.5x11.2MM | Membrane Area = 0.5 cm² | With Mounting Pins

The P2305 Ussing Chamber Slider is designed specifically for physiological studies on larger elongated tissue specimens, such as those from rat, guinea pig, or rabbit intestine. It features a 4.5 x 11.2 mm aperture, offering an expanded 0.5 cm² exposure area. This increased surface area is ideal for studies requiring a broader exposure of tissue, allowing for detailed analysis of transport and barrier functions in larger samples.

Compatible with the EasyMount Chamber Model P2300, the P2305 slider provides a stable and precise platform to support more extensive tissue sections. Its elongated aperture facilitates even and controlled exposure across the entire tissue sample, ensuring consistent and reproducible results. This slider is a valuable tool for researchers conducting high-quality experiments that demand greater surface area, making it suitable for studies involving larger tissue specimens.

Instructions on how to use the P2305 EasyMount Sliders

To use the P2305 Ussing Chamber Slider with the P2300 EasyMount Chamber System, follow these steps to ensure proper setup and reliable experimental results for larger tissue specimens:

1. Prepare the Tissue Sample: Excise the tissue specimen—such as a segment from rat, guinea pig, or rabbit intestine—and carefully trim it to fit the 4.5 x 11.2 mm aperture of the P2305 slider. Ensure the tissue evenly covers the 0.5 cm² exposure area, which is critical for obtaining consistent data across the entire sample.

2. Mount the Tissue on the Slider: Position the tissue over the aperture on the P2305 slider and secure it using the available mounting pins (specific number and location may vary by design). The pins hold the tissue in place without causing unnecessary stress, helping to maintain sample integrity. Confirm that the tissue lies flat and uniformly covers the aperture, as this will ensure optimal exposure and stability during the experiment.

3. Place the Slider into the EasyMount Chamber: After the tissue is mounted, insert the P2305 slider into the P2300 EasyMount Chamber, ensuring proper alignment to create a tight, secure seal. A tight seal is essential for maintaining buffer separation and preventing leaks, ensuring that both sides of the tissue are equally exposed to the buffer solutions.

4. Set Up Experimental Conditions: Fill the Ussing chamber reservoirs with the necessary physiological buffers, adjusting pH and temperature according to the experimental protocol. If required, enable buffer flow or stirring to maintain a consistent environment, which is essential for accurate transport and barrier function measurements.

5. Initiate Experimental Measurements: Begin your assessments, such as transepithelial potential difference (TEPD) or short-circuit current (Isc), depending on the objectives of your study. The P2305’s larger, elongated aperture allows for detailed evaluation of transport properties and barrier integrity across larger tissue areas, facilitating high-quality data collection in studies involving more substantial samples.

6. Post-Experiment Procedures: Once the experiment is complete, carefully remove the slider from the chamber, and gently detach the tissue sample from the mounting pins. Clean the P2305 slider and the P2300 chamber components thoroughly to maintain their condition and ensure readiness for future experiments.

Following these steps will enable you to use the P2305 Ussing Chamber Slider with the P2300 EasyMount Chamber effectively, supporting accurate and reproducible results in studies with larger tissue specimens.