P2305S EasyMount Slider

P2305S EasyMount Slider with Silver Electrodes for Rat, Rabbit, or Guinea Pig Intestine

APETURE SIZE: 4.5x11.2MM | Area = 0.5 cm² | Silver Electrodes for Stimulating Tissue

The P2305S Ussing Chamber Slider is designed specifically for physiological studies on larger elongated tissue specimens, such as those from rat, guinea pig, or rabbit intestine. It features a 4.5 x 11.2 mm aperture, offering an expanded 0.5 cm² exposure area. This increased surface area is ideal for studies requiring a broader exposure of tissue, allowing for detailed analysis of transport and barrier functions in larger samples.

Compatible with the EasyMount Chamber Model P2300, the P2305S slider provides a stable and precise platform to support more extensive tissue sections. Its elongated aperture facilitates even and controlled exposure across the entire tissue sample, ensuring consistent and reproducible results. This slider is a valuable tool for researchers conducting high-quality experiments that demand greater surface area, making it suitable for studies involving larger tissue specimens.

P2305S Ussing Chamber Slider Instruction

To use the P2305S slider in the P2300 EasyMount Chamber System, follow these detailed steps to ensure optimal setup and reliable experimental results:

1. Prepare the Tissue Sample: Carefully excise the tissue specimen, such as mouse intestine, to align with the 4.5 x 11.2 mm aperture of the P2305S slider. Trim and position the tissue to fully cover the 0.5 cm² exposure area, ensuring uniform thickness and smooth placement over the aperture.

2. Mount the Tissue on the Slider: Place the tissue over the aperture on the P2305S slider. Secure it in position using the eight mounting pins, which are specifically designed to hold the sample firmly without inducing mechanical stress or damage. This setup maintains sample integrity, which is essential for achieving consistent results.

3. Set Up the Slider in the EasyMount Chamber: Once the tissue is mounted, insert the P2305S slider into the EasyMount Chamber P2300. Make sure that the slider is properly aligned to create a tight seal within the chamber, preventing buffer leakage and ensuring an even distribution of the buffer solutions on both sides of the tissue.

4. Prepare for Electric Field Stimulation: The P2305S slider includes integrated silver electrodes for transmural electric field stimulation. Connect the electrodes to the appropriate stimulation or measurement equipment. Verify all connections and settings according to your protocol to ensure the electric field is applied consistently across the tissue during the experiment.

5. Conduct the Experiment: Fill the chamber reservoirs with the necessary physiological buffers, maintaining consistent pH and temperature based on experimental requirements. Initiate electric field stimulation and electrophysiological measurements as needed, such as transepithelial potential difference (TEPD) or short-circuit current (Isc), to assess transport properties or barrier function under the influence of electric field modulation.

6. Post-Experiment Handling: After completing the experiment, carefully disassemble the setup by first disconnecting the electrodes and then removing the slider from the chamber. Gently release the tissue from the mounting pins and proceed with any further analyses or sample disposal. Clean and maintain the P2304S slider and chamber components to ensure optimal performance in future experiments.

By following these steps, you can effectively use the P2305S slider within the EasyMount Chamber System to achieve controlled electric field stimulation and high-quality results in studies involving elongated tissue samples. If you need any assistance on using our P2305S slider, please contact us.