P2307D EasyMount Ussing Chamber Slider

P2307D EasyMount Ussing Chamber Slider for Human Duodenum, Colon, and Rectal Biopsies

APETURE SIZE: 0.9MM Circular | Area = 0.006 cm²

The P2307D EasyMount Ussing chamber slider is a highly specialized accessory for the P2300 Ussing Chamber System, expertly designed to support studies involving human biopsy specimens. With its 0.9 mm diameter circular aperture, providing an area of 0.006 cm², the P2307D accommodates small, fragile biopsy samples securely and effectively. It is tailored to hold samples from human tissues, such as duodenal, colonic, and rectal biopsies, which require both precision and gentle handling for accurate physiological measurements.

This slider’s compact circular aperture ensures precise localization for measurements of ion transport, tissue barrier function, and permeability across small biopsy specimens. By concentrating measurements within a 0.006 cm² area, the P2307D minimizes extraneous variables, allowing for high-resolution data specific to the target tissue. The EasyMount design further enhances usability by enabling quick and stress-free sample mounting without invasive pins or additional tools, preserving the integrity of the biopsy and reducing setup time. Used in tandem with the P2300 Chamber, the P2307D slider ensures a stable environment, making it an essential tool for researchers conducting detailed analysis on human epithelial barriers.

Instructions for the P2307D EasyMount Ussing Chamber Slider

To use the P2307D EasyMount Ussing chamber slider in an experiment with the P2300 Ussing Chamber System, researchers should follow a series of steps to ensure proper setup and accurate measurements of physiological parameters in biopsy samples:

1. Sample Preparation

• Begin by preparing a biopsy sample, such as a small section of human duodenum, colon, or rectum. Each tissue sample should be carefully excised to fit within the 0.9 mm circular aperture (0.006 cm²) of the P2307D Slider, ensuring that it aligns well within the slider for consistent exposure and data collection.

2. Mounting the sample on the P2307B Ussing Chamber Slider

• Using the P2307D’s EasyMount design, place the biopsy specimen directly onto the slider’s 0.9 mm aperture, ensuring the tissue is centered and flat over the opening. The EasyMount’s pin-free design is particularly beneficial for preserving the structural integrity of delicate biopsy tissues, minimizing physical stress or damage. This approach also helps streamline mounting, reducing the handling time for samples and allowing quick adjustments.

3. Inserting the Slider into the P2300 Ussing Chamber

• Once the tissue is securely mounted on the P2307D Slider, insert it into the P2300 Ussing Chamber, which has been prepped with suitable buffers or solutions on either side of the chamber. The P2300 Ussing chambers system isolates the tissue into two compartments, typically mimicking luminal and basolateral sides, allowing researchers to simulate physiological conditions or experimental variables.

4. Conducting Measurements

• Connect the electrodes in the P2300 system to measure transepithelial ion transport, potential difference, or other relevant parameters. With the tissue mounted securely on the P2307D Slider, the small, defined area (0.006 cm²) provides a stable measurement zone, ideal for detecting localized transport or permeability changes in biopsy tissue. This setup is particularly effective for studying epithelial barrier function and responses to various treatments or conditions.

5. Data Collection and Analysis

• Maintain stable conditions throughout the experiment, utilizing the P2300’s temperature and aeration controls as needed. The controlled environment of the Ussing Chamber, combined with the P2307D’s precise mounting area, enhances data reproducibility. Data on ion transport, permeability, or drug responses can be continuously monitored, analyzed, and compared across multiple samples to generate reliable and high-resolution insights into the epithelial function of the biopsy tissue.

Using the P2307D Ussing chamber slider with the P2300 Ussing Chamber System provides researchers a high degree of precision and stability, essential for conducting advanced studies on small biopsy samples in a controlled, reproducible environment. If you need any assistance, please contact us.