P2308 EasyMount Ussing Chamber Slider

P2308 EasyMount Ussing Chamber Slider for Very Small Tissues Such As Mouse Pup Trachea & Insect Intestine

APETURE SIZE: 1.0MM Circular | Area = 0.008 cm²

The P2308 EasyMount Ussing chamber slider is a precision tool tailored for use with the P2300 Ussing Chamber System, optimized for very small tissues such as the mouse pup trachea and insect intestine. Featuring a 1.0 mm circular aperture with an area of 0.008 cm², this slider is designed to provide high-resolution measurements from minimal tissue sections. The small aperture size allows researchers to focus on highly localized regions, making it particularly suitable for studies involving delicate tissues where sample size is inherently limited.

Constructed without mounting pins, the P2308 relies on a direct, pressure-fit approach to hold tissues securely in place. This pin-free design reduces the risk of physical distortion or sample stress, preserving the integrity of exceptionally small samples throughout the experiment. In conjunction with the P2300 system, the P2308 Slider offers a stable and reliable platform for measuring ion transport, permeability, and other physiological parameters in minute tissue samples, enhancing data accuracy in high-sensitivity applications.

Instructions for the P2308 Ussing Chamber Slider

To use the P2308 EasyMount Slider in an experiment with the P2300 Ussing Chamber System and a mouse trachea sample, follow these steps to ensure accurate and reproducible results:

1. Tissue Preparation

• Begin by carefully dissecting a section of the mouse trachea small enough to cover the 1.0 mm aperture of the P2308 Slider. Handle the tissue gently to avoid damage, placing it in a physiological solution to maintain viability. This step is crucial to preserve the natural cellular environment and support accurate measurements.

2. Mounting the Tissue on the P2308 Ussing Chamber Slider

• Position the mouse trachea section directly over the 1.0 mm aperture on the P2308 Slider. This slider is designed without mounting pins, so gently secure the tissue by pressing it into place within the aperture area, ensuring it’s flat and centered. The pin-free design minimizes stress, helping preserve the structural and functional integrity of delicate, small tissue samples like the trachea.

3. Inserting the Slider into the P2300 Ussing Chamber

• Insert the loaded P2308 Slider into the P2300 Ussing Chamber. The chamber is typically filled with solutions on both sides to replicate the luminal and basolateral conditions of the trachea, essential for physiological relevance. The P2300 system provides a controlled environment, separating the tissue’s two sides and enabling precise measurement of transport properties or permeability across the tissue barrier.

4. Conducting the Experiment

• Set up the chamber system with electrodes to measure the transepithelial potential difference, resistance, or ion currents across the mounted tissue. The small 0.008 cm² aperture area of the P2308 allows focused, high-resolution data collection from a defined tissue section, ideal for studies on transport function or tissue response in tiny samples. Data on ion flow, permeability, or other physiological responses can be measured as the experiment progresses.

5. Data Collection and Analysis

• Throughout the experiment, monitor conditions closely, adjusting the chamber’s temperature or aeration if needed to maintain stable experimental parameters. The secure mounting and controlled environment provided by the P2300 system and P2308 Slider support high-quality, reproducible data, allowing for detailed analysis of ion transport, barrier function, or pharmacological responses in the mouse trachea tissue.

Using the P2308 Ussing Chamber Slider with the P2300 Ussing Chamber System enables researchers to obtain precise, focused data from small tissue samples, making it an invaluable setup for studies in cellular transport and barrier research in minute, sensitive tissues. If you need any assistance, please contact us.