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The Corning Transwell Snapwell has become a mainstay for growing epithelial cells. Its large area (1.12 cm2) and shallow depth are ideal for transport and electrophysiological research in the EasyMount Ussing chamber. The P2302 EasyMount Slider is designed to securely hold these inserts without stress on the membrane and to position the membrane perfectly between the two halves of the P2300 Ussing chamber.

The mounting procedures for placing the Snapwell into the P2302 slider and then into the chamber are both easy and fast. The Snapwell is removed from the culture dish, separated from its holder, drained of media, and placed with its basal side against the red O-ring in the P2302 slider. Wipe the end and sides of the slider to remove any saline and “slide” the Snapwell+P2302 in the slot between the two chamber halves until it reaches the hard stop. Turn the thumbscrew on the chamber to gently squeeze the chamber halves together. Only light pressure between the chamber halves and the slider is required to make a watertight seal. Excessive pressure can distort the culture membrane and alter the electrical parameters. The procedure for mounting the tissue takes less than 30 seconds to accomplish. This system is ideal for transport and electrophysiological measurements. The P2302 enables effortless transfer of confluent cell layers directly into EasyMount Ussing Chambers, providing researchers with a streamlined, effective solution for high-quality results.