EasyMount Ussing Chamber P2250 - Physiologic Instruments
EasyMount Ussing Chamber P2250 - Physiologic Instruments
EasyMount Ussing Chamber P2250 - Physiologic Instruments
EasyMount Ussing Chamber P2250 - Physiologic Instruments
EasyMount Ussing Chamber P2250 - Physiologic Instruments
EasyMount Ussing Chamber P2250 - Physiologic Instruments
EasyMount Ussing Chamber P2250 - Physiologic Instruments
EasyMount Ussing Chamber P2250 - Physiologic Instruments

P2250 EasyMount Ussing Chamber for Rat Tissues

Designed for Rat Intestinal Tissues

The P2250 Intestinal EasyMount Ussing Chamber is a specialized research tool for studying transport and barrier functions in intestinal tissues, particularly from species like rats, guinea pigs, and rabbits. It features a custom EasyMount Ussing Chamber Slider (P2252) with a 5 x 22 mm oblong aperture, providing a 1.0 cm² area ideal for mounting intestinal samples securely and precisely. With a flexible usable half-chamber volume range of 2.0 to 8.0 ml, the P2250 is adaptable to various experimental protocols. Its design prioritizes durability, ease of use, and reliable data collection, making it a valuable instrument for gastrointestinal research.


⦿ To be used with EasyMount P2252 Chamber Slider Insert

$637.00
$637.00

EasyMount Chamber Details


EasyMount Ussing Chamber P2250 - Physiologic Instruments

P2250 Series EasyMount Ussing Chambers

The P2250 Intestinal EasyMount Ussing Chamber is a specialized tool designed for researchers studying the transport and barrier properties of intestinal tissues, specifically for species such as rats, guinea pigs, and rabbits. Tailored to meet the unique requirements of intestinal research, the P2250 chamber provides an optimal environment for mounting and analyzing delicate tissue samples. Its design ensures that tissue specimens are held securely and positioned accurately, allowing for high-quality, reproducible data that supports a variety of physiological and pharmacological studies focused on gastrointestinal functions.

One of the key features of the P2250 is its custom-designed EasyMount Ussing Chamber Slider, designated as the P2252, which is uniquely suited for mounting intestinal tissues. The P2252 slider insert features an oblong aperture with dimensions of 5 x 22 mm, providing an effective area of 1.0 cm² for the tissue sample. This specific aperture design facilitates the proper alignment and placement of intestinal tissues, enhancing the consistency and reliability of measurements. By using this specialized slider, researchers can achieve a secure and standardized mounting process, critical for accurate assessment of tissue functionality.

The P2250 chamber also offers a flexible volume range, with a usable half-chamber volume of 2.0 to 8.0 ml. This range accommodates various experimental needs, giving researchers the ability to customize conditions based on the requirements of specific protocols. With a durable and easy-to-clean design, the P2250 Ussing Chamber is a practical, long-lasting choice for laboratories focused on intestinal research. Its thoughtful engineering and specialized components make the P2250 an essential tool for advancing studies related to intestinal health, absorption, and barrier properties.

 

P2250 EasyMount Ussing Chamber for Rat Tissues

Key Features:

  • Mounting Intestinal Tissues: This slider is specifically engineered for mounting intestinal tissues and features an oblong aperture measuring 5 x 22 mm, providing an effective area of 1.0 cm² for the tissue sample. The unique aperture design facilitates proper alignment and placement of intestinal tissues, which enhances the consistency and reliability of measurements. By utilizing this specialized slider, researchers can achieve a secure and standardized mounting process, which is critical for accurate assessment of tissue functionality.

  • Flexible Volume Range: The P2250 chamber offers a flexible volume range, with a usable half-chamber volume of 2.0 to 8.0 ml. This versatility accommodates various experimental needs, allowing researchers to customize conditions based on the specific requirements of their protocols. This adaptability is essential for conducting a wide range of experiments in intestinal research.

  • Durable and Easy-to-Clean Design: In addition to its functional features, the P2250 Ussing Chamber is designed for durability and ease of maintenance. Its robust construction ensures longevity, making it a practical choice for laboratories dedicated to intestinal research. The easy-to-clean design further enhances its usability, allowing researchers to maintain a sterile environment for their experiments.

P2250 EasyMount Ussing Chamber

 

The P2250 Intestinal EasyMount Ussing Chamber is an essential tool for advancing studies related to intestinal health, absorption, and barrier properties. Its thoughtful engineering, specialized components, and flexible design make it a valuable asset for researchers focused on understanding gastrointestinal functions. By providing a reliable platform for mounting and analyzing intestinal tissues, the P2250 chamber supports the pursuit of high-quality research and contributes to the advancement of knowledge in the field of intestinal physiology.

 

P2250 EasyMount Ussing Chamber Slider for Rat Tissue

Care and Feeding Instructions for the P2250 EasyMount Chambers


The EasyMount chambers have been machined from high quality acrylic resin. While acrylic is known to be very tough – one might even say “bullet proof” – it is not indestructible and does require some precautions.

The general physical characteristics of acrylics are: Transparency - In colorless form acrylic plastic is as transparent as the finest optical glass. Its total white light transmittance is 92%, the highest transmittance physically possible of any material. Breakage Resistance - Acrylic sheet has from 6 to 17 times greater impact resistance than ordinary glass. It does chip, however, and when dropped onto a hard surface it is likely that the edges will be damaged. Also, because the part has been subjected to stresses during machining, it is possible that a sudden impact will cause the chamber to crack.

Chemical Resistance - Acrylic plastic has excellent resistance to most chemicals, including solutions of inorganic alkalis and acids such as ammonia and sulfuric acid, and aliphatic hydrocarbons such as hexane, octane and VM&P naphtha.

Acrylic is attacked by the following chemicals:
1. Ethyl and methyl alcohol.
2. Chlorinated hydrocarbons such as methylene chloride, a solvent cement widely used, and carbon tetrachloride.
3. Aromatic solvents such as turpentine, benzene, and toluene.
4. Gasoline
5. Organic acids such as acetic acid, phenols, and Lysol.
6. Lacquer thinners and other esters, ketones, and ethers.

The chambers have been annealed after machining to relieve stress in the material and to increase the resistance to attack by these chemicals. They are not, however, impervious and exposure to some of these above may cause the appearance of minute cracks or crazing throughout the material. We recommend that you avoid exposing the chambers to these solvents.

Dimensional Stability - Acrylic sheet is notable for its freedom from shrinking and deterioration through long periods of use.

Combustibility - Acrylic sheet is a combustible thermoplastic and should be treated as an ordinary combustible material such as wood. The self-ignition temperature (spontaneous combustion) of acrylic is between 850 degree F and 869 degree F. The temperature at which the material will ignite in the presence of a flame is between 550 degree F and 570 degree F. Bottom line - the chambers may be damaged by fire or drying them in a hot oven.

Heat Resistance - The maximum "continuous service temperature" of acrylic is about 180 degrees F. Procedures that may increase the temperature above this should be avoided. While the material can withstand higher temperatures for very short periods of time, it will soften and lose its form or shape if subjected to these higher temperatures for any period of time. Procedures to avoid would be placing the chambers in a microwave (the material contains enough moisture to heat considerably), drying the chambers in an oven, and sonicating at high power as this may cause local heating above the melting point. While acrylics respond to heat, they are not affected by cold, and will not become cracked or brittle in cold.

Electrical Properties – The surface resistivity of acrylics is higher than that of most other materials making it a good material for isolating the two sides of an epithelium. U-Factor - heat transfer through acrylic is approximately 20% less than through equivalent thickness of glass.

Cleaning the Chambers: The chambers should be washed in warm water using a mild detergent and then rinsed rigorously with deionized water. We recommend air-drying while placed inverted on a rack or toweling. WARNING: Sonicating the chambers at high energy levels and/or for long times can raise internal temperatures in the acrylic sufficiently to cause melting, deformation of channels, and cracking. 

Cleaning by sonication is, therefore, not recommended.

Note: on stainless steel guide pins: Some of the stainless steel guide pins were made from type 416 stainless. Unfortunately, this type of stainless is less rust resistant than others and we have recently experienced problems with them “rusting” at their tips. Chambers shipped after Oct, 2001 all use type 18-8 stainless, which is more rust resistant and should not present any rust issues. Please contact us if you experience rust problems with any of our chambers.

Although stainless is more resistant to corrosion than mild steel, the name of the metal is a bit misleading. The basic corrosion resistance of stainless steel occurs because of its ability to form a protective coating on the metal surface due to the combining of oxygen with the chrome in the stainless to form chrome oxide (commonly called ceramic). This coating is a passive film that prevents oxidation of the iron in the steel (rusting). Unfortunately, this film is subject to attack by halogen salts, and chlorides are especially good at penetrating this passive film. Once broken, corrosive attack or “rusting” can occur. Since most physiological saline solutions contain chloride the guide pins are frequently exposed to chloride salts. Also, sterilizing the chamber with Clorox bleach will expose the pins to high concentrations of halide. We suggest routinely protecting the pins as follows. Firstly, remove any rust spots from the pins using a brass, silver, or chrome cleaner. Then, after normal use, rinse the ins with distilled water and dry them by wiping with a cloth containing a little silicone car wax. This will protect them and make them slide easily into the chamber.