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Sistemas de Cámaras Ussing para la Investigación de la Barrera Epitelial  |        Servicio al Cliente en EE. UU.: +1-858-451-8845   |        Email: office@piusa.net
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Ussing Chamber: The Complete Guide (Principles, Protocols, Troubleshooting & Product Selector)

Ussing Chamber: The Complete Guide

This lab-ready guide explains how Ussing chambers quantify epithelial transport, provides reproducible short-circuit current (SCC) and TEER workflows, and helps you choose the right system from Physiologic Instruments for consistent, publication-quality data.

What is an Ussing Chamber?

An Ussing chamber measures transepithelial ion transport across cell or tissue preparations by clamping voltage and recording current (SCC) or by measuring resistance (TEER).

Behind the science: In SCC mode, tissue is held at 0 mV and the amplifier injects current to oppose the native transepithelial potential; this injected current is proportional to ion transport.

Core Components

Chambers & Sliders

Hold tissue/inserts and define aperture & volume.

Electrodes

Ag/AgCl voltage-sensing + current-passing pairs.

Amplifier / Clamp

Multichannel control for SCC/TEER.

Perfusion & Heating

Maintain temperature, gas, and solution exchange.

When to Use an Ussing Chamber

  • CFTR and ENaC function studies
  • Barrier integrity via TEER
  • Drug transport and permeability
  • Time- and dose-response kinetics

Validated SCC Protocol

  1. Prep buffers and electrodes
  2. Mount tissue, confirm no leaks
  3. Clamp at 0 mV, record SCC
  4. Apply sequence: amiloride → cAMP agonist → inhibitor
  5. Check quality gates and export data

Quick TEER Workflow

  1. Apply a small V step
  2. Compute R = ΔV / ΔI, area corrected
  3. Trend TEER over time

Data Quality Checklist

  • Matched electrodes
  • Stable temperature
  • No bubbles crossing aperture
  • Fresh reagents, matched osmolarities

Troubleshooting Matrix

Symptom Likely Cause Fix
Unstable baseline Offsets/bubbles Re-seat electrodes, degas buffers
Low SNR Grounding issues Improve earth/line routing
No inhibitor-sensitive current Reagent/sequence error Check order, concentrations

Product Selector

Use Case System Benefits Link
Cell monolayers EM8-C EasyMount Broad compatibility View
Low volumes EM8-LV Faster equilibration View
Rat tissue EM8-R Specialized tooling View
8-channel VCC MC8 Clamp Parallel SCC/TEER View

FAQs

What aperture should I choose?

Match aperture to tissue thickness and current.

How do I avoid drift?

Stabilize temperature, avoid bubbles, equilibrate.

Can I run SCC and TEER on the same prep?

Yes, run SCC under clamp and pulse for TEER.

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