P2302T₁₂ EasyMount Slider

P2302T₁₂ EasyMount Slider for Corning-Costar Transwell Inserts

APETURE SIZE: 12MM | Area = 1.12 cm²

The P2302T₁₂ EasyMount Slider offers an efficient, precision-engineered solution for integrating 12 mm Corning-Costar Transwell Inserts with an aperture area of 1.12 cm². Designed to meet the demands of high-sensitivity assays, such as advanced drug transport, permeability, and cellular barrier studies, this slider provides a stable, secure fit for Transwell Inserts, enabling straightforward assembly and optimal positioning in our EasyMount Ussing chambers. Its versatility supports both vertical and horizontal experimental setups, making it an invaluable tool for labs conducting rigorous cell culture experiments. The P2302T₁₂ EasyMount Slider streamlines complex workflows, providing reproducible, reliable data, and enhancing experimental accuracy for scientists focused on cellular function and transport research.

P2302T₁₂ Sliders with Transwell Inserts Instructions:

Inserting a Corning-Costar Transwell Insert into the Physiologic Instruments P2302T₁₂ EasyMount Slider involves several precise steps to ensure proper alignment and stability for your experiments. Here’s how to do it effectively:

1. Prepare the Transwell Insert: Start with a pre-cultured Transwell insert that’s ready for placement into the EasyMount slider. Make sure it has the correct dimensions (e.g., a 6.5 mm for the P2302T or a 12 mm diameter insert from the P2302T₁₂) that matches the P2302T₁₂ EasyMount Slider aperture. Keep it in a sterile environment if necessary to maintain the sample’s integrity.

2. Assemble the EasyMount Slider: The P2302T₁₂ EasyMount Slider comes with a specialized opening to accommodate the Transwell insert. With the slider positioned on a clean, flat surface, make sure the aperture in the slider is free of any obstructions, as this will be the site where the Transwell insert is secured.

   NOTE: Dry the inside chamber walls and the rear heat block with a tissue (must be dry before inserting slider into chamber.
   

3. Insert the Transwell into the Slider Aperture: Carefully align the Transwell insert with the aperture in the slider. Gently press the insert down into the aperture until it securely fits into place. The design of the P2302T₁₂ slider provides a snug fit, which stabilizes the insert, ensuring that it will remain in the correct position throughout the experiment. Avoid excessive force to prevent damaging the membrane or altering the cell culture.

   NOTE: It requires the Transwell hanger to be cut away during mounting.
   

4. Secure the Transwell Insert with the Locking Mechanism: Depending on the specific model of the P2302T₁₂ slider, there may be a locking ring or clamp to fully secure the Transwell in place. If the slider has this feature, engage it by pressing or tightening it until you feel a slight resistance, confirming that the insert is fully immobilized. This ensures the Transwell will not shift during handling or within the Ussing chamber.

5. Place the Assembled Slider into the Chamber: With the Transwell insert now securely mounted in the EasyMount Slider, position the entire assembly into your Ussing or diffusion chamber according to your setup requirements. The EasyMount Slider is designed for compatibility with both vertical and horizontal orientations, offering flexible positioning based on your experimental needs.

This setup will provide a stable and precise environment for studies on transport, permeability, and cellular barrier integrity. By securing the Transwell in the EasyMount Slider, you ensure consistent, reproducible results while maintaining easy access and handling for your cell culture insert.