P2400 EasyMount Low Volume Ussing System for Cell & Tissue
The P2400 EasyMount Low Volume System is designed for the study of transport in tissues where low net transport quantities or assays requiring higher solute concentrations are involved. This system accommodates various tissue sizes and operates within a low-volume chamber environment.
The system includes the EasyMount base unit, P2400 low-volume chambers (with working volumes ranging from 0.8 mL to 5 mL per chamber half), and a selection of tissue sample holders (one per chamber). A range of holders is available, supporting small tissue samples such as human intestinal or nasal mucosal biopsies, mouse pup intestine, cornea, and larger tissues up to 0.71 cm² (9.5 mm diameter). Custom holders can be fabricated to suit specific tissue requirements. Note that P2400 series sliders are sold separately from the system.
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Product Description
P2400 EasyMount Low Volume Ussing System for Cell & Tissue
The Low Volume System is used to study transport in tissues where the net quantity transported is low or the assay for the transported solute requires higher concentrations. This system, like the system for Snapwell culture inserts above, permits a variety of tissue sizes to be mounted in a low volume chamber.
The system includes the basic EasyMount system described above, P2400 low volume chambers (working volume 0.8 ml to 5 ml per chamber half) and choice of holders for mounting tissue samples (1 per chamber). Several different holders are available for these chambers including those for small tissue samples such as human intestinal or nasal mucosal biopsy, mouse pup intestine, cornea, etc. as well as tissues up to 0.71 cm2 (9.5 mm diameter). Special holders can be made to accommodate your specific tissue. P2400 series sliders are to be purchased separately from system.
The EM8-LV Low Volume Ussing System Includes:
| Quantity | Voltage/Current Clamp Parts | Quantity | Software | |
| 1 | VCC MC8-8S voltage/current clamp with optiGain | 1 | Acquire & Analyze Data Acquisition package with DI-720P | |
| 8 | DM-MC6 Input Modules with Dummy Membrane | |||
| Quantity | Chamber Parts | Quantity | Accessories | |
| 1 | EM-LVSYS-8 Chamber System | 16 | FN15 Filling Needles | |
| 8 | P2400 Chambers | 1 | GWB1 Gas Washing Bottle (1 L) | |
| 8 | P2020-S Electrode sets | 1 | TK-7 Tubing and Setup Kit | |
| 8 | P2024-40 Electrode Lead Sets | |||
| 8 | P2060 Air Valve Sets | |||
| 8 | P2400-Caps (chamber caps) |
A/D Converter System does not include sliders as these are specific to your experiments; Up close with our Ussing Chamber & Stand Anodized Aluminum Heat Block with Cradles Precision Acrylic Ussing Chamber with Electrodes Chamber Caps & Adjustable Air Valves Ideal gas lift circulation Used to measure the net ion transport taking place across the epithelium
A voltage is generated across the epithelium as a consequence of ion selective channels in the membranes and active transport. This voltage is sensed by the black Ag/AgCl electrodes and measured by the voltage clamp instrument. Current may be passed by the clamp instrument across the epithelium via the white electrodes. When this current is sufficient to exactly cancel the voltage generated by the epithelium, the epithelium is “short-circuited” and the current necessary to do this is the short-circuit current (Isc).
When the solutions in the chamber compartments are the same, the Isc is a measure of the net active transport of ions across the epithelium. VCC MC8-8S voltage current clamp optiGain for switching current gain and fluid resistance ranges Input Modules (DM MC6) & Dummy Membrane8-Channels for each chamber Pulse Generator Master Control Acquire & Analyze Data Acquisition Specifically for measuring bioelectrics from small pieces of epithelial tissue or cells Data Acquisition Hardware Interface cables ACQUIRE and ANALYZE software runs on Windows® to measure current, voltage, conductance and resistance from one to eight tissues simultaneously.
Low Volume EasyMount Ussing System options:
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Published: 18 June 2024 |
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Published: 29 February 2024 |
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Published: 21 May 2024 - Thematic Poster Session / San Diego Convention Center |
