Chamber Components

The Corning Transwell Snapwell has become a mainstay for growing epithelial cells. Its large area (1.12 cm2) and shallow depth are ideal for transport and electrophysiological research in the EasyMount Ussing chamber. The P2302 EasyMount Slider is designed to securely hold these inserts without stress on the membrane and to position the membrane perfectly between the two halves of the P2300 Ussing chamber.

The mounting procedures for placing the Snapwell into the P2302 slider and then into the chamber are both easy and fast. The Snapwell is removed from the culture dish, separated from its holder, drained of media, and placed with its basal side against the red O-ring in the P2302 slider. Wipe the end and sides of the slider to remove any saline and “slide” the Snapwell+P2302 in the slot between the two chamber halves until it reaches the hard stop. Turn the thumbscrew on the chamber to gently squeeze the chamber halves together. Only light pressure between the chamber halves and the slider is required to make a watertight seal. Excessive pressure can distort the culture membrane and alter the electrical parameters. The procedure for mounting the tissue takes less than 30 seconds to accomplish. This system is ideal for transport and electrophysiological measurements. The P2302 enables effortless transfer of confluent cell layers directly into EasyMount Ussing Chambers, providing researchers with a streamlined, effective solution for high-quality results.

Achieve accurate electrophysiological studies with the P2302M EasyMount Ussing Chamber Slider, featuring a aperture of 8.75mm perfect for millicell cell culture inserts.

Our P2302M EasyMount Ussing Chamber Slider is the right setup when using Millicell® cell culture inserts in an ussing chamber. With this slider you can simulate the three-dimensional environment for natural, multidimensional cell growth. Access both surfaces of cell layers for more relevant in vitro models and accurate results. Perfect for toxicity screening, primary and secondary screening, and cell signaling studies.

Optimize your transport and electrophysiological studies with the P2302N EasyMount Ussing Chamber Slider. Featuring an advanced Nunc/Anapore polycarbonate culture insert with an 8 mm tissue-culture-treated membrane supported by a removable ring, this device ensures stable, adaptable positioning in EasyMount ussing chambers. The P2302N EasyMount Slider is an essential tool for enhancing research efficiency and precision.

The P2302T EasyMount Ussing Chamber Slider is specifically designed to provide unmatched convenience and precision for researchers using Corning-Costar Transwell Inserts. With a 6.5 mm aperture size and an area of 0.33 cm², this slider is ideal for a wide range of cellular assays, from barrier function and permeability studies to drug transport analysis.

Ensuring a secure, stable fit for Transwell Inserts, the P2302T slider is making it easy to set up and providing consistent, reproducible results. Its compatibility with diffusion and Ussing chambers enhances experimental flexibility, allowing seamless integration into both vertical and horizontal setups.

The P2252 EasyMount Slider has an oblong 4.8 x 22 mm aperture and is designed for mounting intestinal tissues with a surface area of no less than 1 cm² and for use with EasyMount P2250 Chambers. Composed of a top clear and bottom white segment. Tissues are mounted by pressing the tissue over short, curved pins lining a matching aperture on the slider.

The aperture has a surrounding recess to minimize edge-effect caused by minimal compression from mounting. The large surface area is useful when performing ion flux or drug permeability studies. The large surface area also maximizes detecting endogenous peptide secretion from the lumen or from the basolateral side.

The P2250 Intestinal EasyMount Ussing Chamber is a specialized research tool for studying transport and barrier functions in intestinal tissues, particularly from species like rats, guinea pigs, and rabbits. It features a custom EasyMount Ussing Chamber Slider (P2252) with a 5 x 22 mm oblong aperture, providing a 1.0 cm² area ideal for mounting intestinal samples securely and precisely. With a flexible usable half-chamber volume range of 2.0 to 8.0 ml, the P2250 is adaptable to various experimental protocols. Its design prioritizes durability, ease of use, and reliable data collection, making it a valuable instrument for gastrointestinal research.

The P2300 EasyMount Ussing Chamber is designed as a versatile and reliable instrument for experimental research focused on transport and barrier functions of biological tissues and cell monolayers. It incorporates an intuitive design that accommodates a broad range of sample types, from native animal tissues to cultured cell layers on permeable supports, with straightforward integration into both standard and customized laboratory workflows. Equipped with precision-engineered EasyMount sliders, the P2300 simplifies the setup and alignment of samples, minimizing handling time and enhancing reproducibility, crucial for consistent results across experiments.

The chamber’s flexibility in half-chamber volume (2.0–8.0 ml) allows researchers to tailor the setup to specific experimental needs, optimizing resources while maintaining accuracy. With durable construction materials suited for frequent use and compatibility with various physiological measurement devices, the P2300 is not only adaptable but also built to last, supporting the rigorous demands of high-quality research in physiology, pharmacology, and related fields.

The P2400 Low-Volume EasyMount Ussing Chamber is tailored for studies involving animal tissues, specifically designed to optimize experiments requiring minimal reagent volumes. With a usable half-chamber volume of 1.0 to 5.0 ml, it is ideal for research using expensive drug solutions or FLUX measurements. The P2400 uses custom EasyMount sliders for precise and secure tissue mounting, accommodating various tissue types for versatile applications. Sold in pairs for efficient experimental setups, the P2400 offers durability, ease of use, and cost-saving benefits, making it a valuable tool for precise, low-volume physiological studies.

The P2302T₁₂ EasyMount Ussing Chamber Slider offers an efficient, precision-engineered solution for integrating 12 mm Corning-Costar Transwell Inserts with an aperture area of 1.12 cm². Designed to meet the demands of high-sensitivity assays, such as advanced drug transport, permeability, and cellular barrier studies, this slider provides a stable, secure fit for Transwell Inserts, enabling straightforward assembly and optimal positioning in our EasyMount Ussing chambers.

Its versatility supports both vertical and horizontal experimental setups, making it an invaluable tool for labs conducting rigorous cell culture experiments. The P2302T₁₂ EasyMount Slider streamlines complex workflows, providing reproducible, reliable data, and enhancing experimental accuracy for scientists focused on cellular function and transport research.

Introducing the P2303 Ussing Chamber Slider: an advanced, precision-engineered solution tailored to elevate the accuracy and efficiency of physiological research on small tissue samples, including delicate specimens like mouse intestine. Featuring a precise 2.7 x 4 mm aperture, the P2303 provides an optimized 0.10 cm² exposure area specifically designed to meet the demands of small-scale studies.

The P2303 slider sets a new standard by eliminating traditional mounting pins, a common source of stress and potential damage to fragile tissues. This advanced, pin-free design preserves sample integrity and enhances the reliability of experimental outcomes by reducing mechanical impact, making it an essential tool for achieving consistent, high-quality results in physiological research.

The P2303A Ussing Chamber Slider from Physiologic Instruments is precisely designed to support high-precision physiological studies on small tissue specimens, including mouse intestine and bladder. With a 2.7 x ~4 mm aperture and an optimized 0.10 cm² exposure area, this specialized slider offers a refined platform that enhances experimental accuracy and reproducibility for delicate samples. Its design ensures that tissues receive uniform exposure, contributing to more reliable data in studies focused on transport and barrier function.

This slider is compatible with the EasyMount Chamber Model P2300 and incorporates six mounting pins for secure sample stabilization without compromising tissue integrity. The P2303A’s robust yet sensitive configuration allows researchers to handle and maintain small, fragile tissues with confidence, reducing mechanical stress and improving the quality of results. Tailored to the needs of specialized physiological research, the P2303A Ussing Chamber Slider exemplifies advanced engineering for laboratory environments demanding precision and dependability.

The P2304 Ussing Chamber Slider is purposefully engineered for advanced physiological studies involving elongated tissue specimens, including mouse intestine sections. With a 2.7 x 11.2 mm aperture providing a 0.3 cm² exposure area, this slider is optimized to deliver precise, reliable results for assessing transport and barrier functions in extended tissues.

Designed to integrate seamlessly with the EasyMount Chamber Model P2300, the P2304 slider includes eight mounting pins that secure the tissue in place, minimizing sample movement and mechanical stress. This added stability preserves sample integrity, ensuring the accuracy and consistency of experimental data. The P2304 Ussing Chamber Slider is an ideal choice for researchers seeking high-quality, reproducible results in studies requiring controlled exposure of elongated tissue samples.

The P2304S Ussing Chamber Slider is specifically engineered for advanced physiological studies involving elongated tissue specimens, such as mouse intestine sections. Featuring a 2.7 x 11.2 mm aperture with an exposure area of 0.3 cm², this slider offers a precise platform for examining transport and barrier functions in extended tissues, ensuring reliable and reproducible data across experiments.

This special chamber slider is equipped with integrated silver electrodes to enable controlled transmural electric field stimulation, supporting studies that require precise electrical modulation across tissue layers. Compatible with the EasyMount Chamber Model P2300, the P2304S slider provides a robust solution for researchers conducting complex studies on small, elongated tissue samples, enhancing both experimental control and data fidelity.

The P2305 Ussing Chamber Slider is purpose-built for physiological studies involving larger, elongated tissue specimens, such as those from rat, guinea pig, or rabbit intestine. With a 4.5 x 11.2 mm aperture and a 0.5 cm² exposure area, this slider provides an expanded surface optimized for examining transport and barrier functions in larger tissue samples, supporting detailed and reproducible analysis.

Designed for seamless compatibility with the EasyMount Chamber Model P2300, the P2305 slider offers a stable and precise platform for securely positioning extended tissue sections. The elongated aperture ensures consistent exposure across the sample, enhancing data reliability and facilitating controlled studies. The P2305 Ussing Chamber Slider is an ideal choice for researchers requiring a larger exposure area for rigorous experiments on substantial tissue specimens.

The P2305S Ussing Chamber Slider is purpose-built for physiological studies involving larger, elongated tissue specimens, such as those from rat, guinea pig, or rabbit intestine. With a 4.5 x 11.2 mm aperture and a 0.5 cm² exposure area, this slider provides an expanded surface optimized for examining transport and barrier functions in larger tissue samples, with a supporting silver electrode built into the slider.

Designed for seamless compatibility with the EasyMount Chamber Model P2300, the P2305S slider offers a stable and precise platform for securely positioning extended tissue sections. The elongated aperture ensures consistent exposure across the sample, enhancing data reliability and facilitating controlled studies. The P2305S Ussing Chamber Slider is an ideal choice for researchers requiring a larger exposure area for rigorous experiments on substantial tissue specimens.

The P2306 Ussing Chamber Slider offers a solution for researchers working with small, delicate tissue samples, such as mouse trachea, in high-resolution electrophysiological studies. With a compact 2.8 x 1.5 mm frame and a carefully calibrated aperture area of 0.040 cm², this chamber slider enables researchers to obtain accurate, controlled measurements of ion transport, tissue permeability, and other physiological parameters from a precisely defined tissue area. Designed to integrate seamlessly with the EasyMount P2300 Chamber system, the P2306 Slider supports stable, reproducible experimental conditions essential for in-depth tissue analysis and consistent data collection, ultimately enhancing the quality of your research outcomes.

The P2307 EasyMount Ussing Chamber Slider is a precise and efficient accessory for the P2300 Ussing Chamber System, ideal for human biopsy studies, including duodenal, colonic, and rectal samples. Its 2 mm diameter circular aperture, covering a focused area of 0.031 cm², allows for detailed measurement of ion transport and barrier function within small, targeted tissue sections.

The EasyMount design facilitates quick, secure mounting without pins, preserving sample integrity. In combination with the P2300 system, the P2307 Slider ensures stable and reproducible conditions, making it a valuable tool for high-resolution epithelial research on biopsy specimens.